Sequencing By Synthesis
Sequencing by synthesis (SBS) is one of the new generation mass sequencing technologies that use clonal amplification in vitro by means of a bridge PCR.
The fragments to be sequenced are prepared with adapters at the ends to allow their denatured strands to bind to a solid surface and thus be enriched by means of a bridge-type amplification. This generates clusters of strands of the same fragment that amplifies the signal of the fluorescent nucleotides as they are incorporated into the growing chain.
